rabbit anti-als2 Search Results


anti rabbit alsin  (Novus Biologicals)
90
Novus Biologicals anti rabbit alsin
BAC GFP-Rab5 cells seeded on a gridded dish were labeled with MitoTracker-Red CMSRox and photoirradiated as before. Cells were fixed after 30 min post-laser treatment and immunostained with specific ZFYVE20 and EEA1 ( A ), and APPL1 and APPL2 antibodies ( B ). ( C ) Quantification of colocalization from untreated and laser-treated cells in ( A ) and ( B ), n = 3. ( D ) Quantification of colocalization between Rab5 and EEA1 as shown in ( A ), n = 3. ( E - F ) BAC GFP-Rab5 cells were treated and processed in the same manner as in ( A ) and immunostained with specific Rabex-5 and <t>Alsin</t> antibodies, respectively. ( G ) Quantification of colocalization from untreated and laser-treated cells in ( E ) and ( F ), n = 3. Scale bars, 10 μm.
Anti Rabbit Alsin, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti rabbit alsin/product/Novus Biologicals
Average 90 stars, based on 1 article reviews
anti rabbit alsin - by Bioz Stars, 2026-03
90/100 stars
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rabbit anti als2  (Proteintech)
93
Proteintech rabbit anti als2
BAC GFP-Rab5 cells seeded on a gridded dish were labeled with MitoTracker-Red CMSRox and photoirradiated as before. Cells were fixed after 30 min post-laser treatment and immunostained with specific ZFYVE20 and EEA1 ( A ), and APPL1 and APPL2 antibodies ( B ). ( C ) Quantification of colocalization from untreated and laser-treated cells in ( A ) and ( B ), n = 3. ( D ) Quantification of colocalization between Rab5 and EEA1 as shown in ( A ), n = 3. ( E - F ) BAC GFP-Rab5 cells were treated and processed in the same manner as in ( A ) and immunostained with specific Rabex-5 and <t>Alsin</t> antibodies, respectively. ( G ) Quantification of colocalization from untreated and laser-treated cells in ( E ) and ( F ), n = 3. Scale bars, 10 μm.
Rabbit Anti Als2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti als2/product/Proteintech
Average 93 stars, based on 1 article reviews
rabbit anti als2 - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
rabbit anti-als2  (Millipore)
90
Millipore rabbit anti-als2
BAC GFP-Rab5 cells seeded on a gridded dish were labeled with MitoTracker-Red CMSRox and photoirradiated as before. Cells were fixed after 30 min post-laser treatment and immunostained with specific ZFYVE20 and EEA1 ( A ), and APPL1 and APPL2 antibodies ( B ). ( C ) Quantification of colocalization from untreated and laser-treated cells in ( A ) and ( B ), n = 3. ( D ) Quantification of colocalization between Rab5 and EEA1 as shown in ( A ), n = 3. ( E - F ) BAC GFP-Rab5 cells were treated and processed in the same manner as in ( A ) and immunostained with specific Rabex-5 and <t>Alsin</t> antibodies, respectively. ( G ) Quantification of colocalization from untreated and laser-treated cells in ( E ) and ( F ), n = 3. Scale bars, 10 μm.
Rabbit Anti Als2, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-als2/product/Millipore
Average 90 stars, based on 1 article reviews
rabbit anti-als2 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
rabbit anti als2 polyclonal antibody  (Cusabio)
N/A
Rabbit anti Human ALS2 Polyclonal Antibody
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rabbit anti als2cr11 polyclonal antibody  (Cusabio)
N/A
Rabbit anti Human ALS2CR11 Polyclonal Antibody
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rabbit anti lgals2 polyclonal antibody  (Cusabio)
N/A
Rabbit anti Human LGALS2 Polyclonal Antibody
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als2cr1 nif3l1 rabbit polyclonal antibody  (OriGene)
N/A
Rabbit Polyclonal Anti NIF3L1 Antibody
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als2cr8 antibody  (ProSci Incorporated)
N/A
ALS2CR8 Antibody raised in Rabbit validated in WB in Human
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rabbit anti human stradb als2cr2 aa200 250  (RayBiotech)
N/A
STRADB ALS2CR2 Rabbit anti Human Polyclonal aa200 250 Unconjugated Antibody 50 µg
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anti als2cr11 antibody  (St Johns Laboratory)
N/A
Rabbit polyclonal to ALS2CR11 Conjugation note Unconjugated Application note WB ELISA Reactivity note Human
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galectin 2 lgals2 rabbit polyclonal antibody  (OriGene)
N/A
Anti LGALS2 Rabbit Polyclonal Antibody
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als2cr12 antibody [dylight 350]  (Novus Biologicals)
N/A
The ALS2CR12 Antibody [DyLight 350] from Novus is a ALS2CR12 antibody to ALS2CR12. This antibody reacts with Human. The ALS2CR12 antibody has been validated for the following applications: Western Blot, Immunocytochemistry/ Immunofluorescence.
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Image Search Results


BAC GFP-Rab5 cells seeded on a gridded dish were labeled with MitoTracker-Red CMSRox and photoirradiated as before. Cells were fixed after 30 min post-laser treatment and immunostained with specific ZFYVE20 and EEA1 ( A ), and APPL1 and APPL2 antibodies ( B ). ( C ) Quantification of colocalization from untreated and laser-treated cells in ( A ) and ( B ), n = 3. ( D ) Quantification of colocalization between Rab5 and EEA1 as shown in ( A ), n = 3. ( E - F ) BAC GFP-Rab5 cells were treated and processed in the same manner as in ( A ) and immunostained with specific Rabex-5 and Alsin antibodies, respectively. ( G ) Quantification of colocalization from untreated and laser-treated cells in ( E ) and ( F ), n = 3. Scale bars, 10 μm.

Journal: bioRxiv

Article Title: Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria

doi: 10.1101/200428

Figure Lengend Snippet: BAC GFP-Rab5 cells seeded on a gridded dish were labeled with MitoTracker-Red CMSRox and photoirradiated as before. Cells were fixed after 30 min post-laser treatment and immunostained with specific ZFYVE20 and EEA1 ( A ), and APPL1 and APPL2 antibodies ( B ). ( C ) Quantification of colocalization from untreated and laser-treated cells in ( A ) and ( B ), n = 3. ( D ) Quantification of colocalization between Rab5 and EEA1 as shown in ( A ), n = 3. ( E - F ) BAC GFP-Rab5 cells were treated and processed in the same manner as in ( A ) and immunostained with specific Rabex-5 and Alsin antibodies, respectively. ( G ) Quantification of colocalization from untreated and laser-treated cells in ( E ) and ( F ), n = 3. Scale bars, 10 μm.

Article Snippet: Cells were immunostained with corresponding primary: anti-rabbit Rabenosyn-5/ZFYVE20 (Sigma Aldrich: HPA044878), anti-mouse EEA1 (BD Biosciences: 610457), anti-rabbit TOM20 (Santa Cruz Biotechnology: sc-11415), anti-rabbit APPL1 (Abcam: ab59592), anti-mouse Rab5 (BD Biosciences: 610724), anti-mouse cytochrome c (Abcam: ab6311), and anti-rabbit Alsin (Novus Biological: NBP2-14284) antibodies.

Techniques: Labeling

( A ) BAC GFP-Rab5 cells were seeded on glass coverslip, fixed, and immunostained with specific antibodies against Rabex-5. ( B ) A schematic representation of domain organization of full-length human Alsin. The regulator of chromosome condensation 1 (RCC1), B-cell lymphoma (Dbl) homology (DH) and pleckstrin homology (PH), membrane occupation and recognition nexus (MORN) motif, and vacuolar protein sorting 9 (VPS9) domains are labeled. Three putative GEF domains are annotated. ( C ) Immunostaining of HeLa cells with specific antibodies against Rab5 and Alsin.

Journal: bioRxiv

Article Title: Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria

doi: 10.1101/200428

Figure Lengend Snippet: ( A ) BAC GFP-Rab5 cells were seeded on glass coverslip, fixed, and immunostained with specific antibodies against Rabex-5. ( B ) A schematic representation of domain organization of full-length human Alsin. The regulator of chromosome condensation 1 (RCC1), B-cell lymphoma (Dbl) homology (DH) and pleckstrin homology (PH), membrane occupation and recognition nexus (MORN) motif, and vacuolar protein sorting 9 (VPS9) domains are labeled. Three putative GEF domains are annotated. ( C ) Immunostaining of HeLa cells with specific antibodies against Rab5 and Alsin.

Article Snippet: Cells were immunostained with corresponding primary: anti-rabbit Rabenosyn-5/ZFYVE20 (Sigma Aldrich: HPA044878), anti-mouse EEA1 (BD Biosciences: 610457), anti-rabbit TOM20 (Santa Cruz Biotechnology: sc-11415), anti-rabbit APPL1 (Abcam: ab59592), anti-mouse Rab5 (BD Biosciences: 610724), anti-mouse cytochrome c (Abcam: ab6311), and anti-rabbit Alsin (Novus Biological: NBP2-14284) antibodies.

Techniques: Membrane, Labeling, Immunostaining

( A ) Electrophoresis result of the PCR reaction using primers flanking exon 3 and within exon 3. Homozygous deletion was confirmed by the absence of a 2.8 kb in Alsin -/- . ( B ) Protein lysates from WT KOLF and Alsin -/- iPSC were loaded onto SDS-PAGE and probed with antibody against Alsin. A band detected at ∼184 kDa, but absent in Alsin -/- , corresponds to full-length Alsin. ( C ) WT KOLF and Alsin -/- iPSC and neuroprogenitor cells (NPC) were immunostained with pluripotency markers Oct4 and Lin28, and neuroprogenitor markers Sox2 and Pax6, respectively. ( D-E ) WT KOLF and Alsin -/- iPSC-sMN were immunostained with motor neuron markers such as ChAT, HB9, and ISL1, nuclear dye DAPI, and cytoskeletal marker MAP2. ( F ) WT KOLF and Alsin -/- iPSC-sMN were immunostained with specific antibodies against Rab5 and Alsin, along with DAPI (nuclear) and phalloidin (actin) stains. Scale bars, 10 μm.

Journal: bioRxiv

Article Title: Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria

doi: 10.1101/200428

Figure Lengend Snippet: ( A ) Electrophoresis result of the PCR reaction using primers flanking exon 3 and within exon 3. Homozygous deletion was confirmed by the absence of a 2.8 kb in Alsin -/- . ( B ) Protein lysates from WT KOLF and Alsin -/- iPSC were loaded onto SDS-PAGE and probed with antibody against Alsin. A band detected at ∼184 kDa, but absent in Alsin -/- , corresponds to full-length Alsin. ( C ) WT KOLF and Alsin -/- iPSC and neuroprogenitor cells (NPC) were immunostained with pluripotency markers Oct4 and Lin28, and neuroprogenitor markers Sox2 and Pax6, respectively. ( D-E ) WT KOLF and Alsin -/- iPSC-sMN were immunostained with motor neuron markers such as ChAT, HB9, and ISL1, nuclear dye DAPI, and cytoskeletal marker MAP2. ( F ) WT KOLF and Alsin -/- iPSC-sMN were immunostained with specific antibodies against Rab5 and Alsin, along with DAPI (nuclear) and phalloidin (actin) stains. Scale bars, 10 μm.

Article Snippet: Cells were immunostained with corresponding primary: anti-rabbit Rabenosyn-5/ZFYVE20 (Sigma Aldrich: HPA044878), anti-mouse EEA1 (BD Biosciences: 610457), anti-rabbit TOM20 (Santa Cruz Biotechnology: sc-11415), anti-rabbit APPL1 (Abcam: ab59592), anti-mouse Rab5 (BD Biosciences: 610724), anti-mouse cytochrome c (Abcam: ab6311), and anti-rabbit Alsin (Novus Biological: NBP2-14284) antibodies.

Techniques: Electrophoresis, SDS Page, Marker

( A ) Flow chart depicting the different stages and time (in days) from iPSC, to neuroprogenitor cells (NPC), and to generating mature spinal motor neurons (sMN). Figure legends indicate the various small molecules and compounds that are used at different stages. ( B ) WT and Alsin -/- cells were challenged with 100 μM H 2 O 2 for 1 hour. Cells were fixed and immunostained with Rab5 and TOM20 antibodies. Inset images show the representative of the signals from TOM20 and Rab5 under control (left panel) and H 2 O 2 -treated condition (right panel). Scale bars, 10 μm. ( C ) Subcellular fractionation of cytosolic (Cyto) and mitochondrial (Mito) fractions from WT and Alsin -/- iPSC-sMN treated with either PBS (control) or 200 μM H 2 O 2 for 1 hours at 37°C. Protein samples were loaded onto SDS-PAGE and imunoblotted with Rab5, tubulin (cytoplasmic loading control), and TOM20 antibodies by Western blot. ( D ) Cytosolic fractions were prepared as in from WT and Alsin -/- iPSC-sMN challenged with 200 μM H 2 O 2 for 1 hours at 37μC. Densitometric quantification of cytosolic cytochrome c from Western blot probed cytochrome c antibody. Data were collected from three independent experiments. Y-axis corresponds to normalized ratio intensity of experimental to loading control (* p <0.05; ** p <0.005).

Journal: bioRxiv

Article Title: Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria

doi: 10.1101/200428

Figure Lengend Snippet: ( A ) Flow chart depicting the different stages and time (in days) from iPSC, to neuroprogenitor cells (NPC), and to generating mature spinal motor neurons (sMN). Figure legends indicate the various small molecules and compounds that are used at different stages. ( B ) WT and Alsin -/- cells were challenged with 100 μM H 2 O 2 for 1 hour. Cells were fixed and immunostained with Rab5 and TOM20 antibodies. Inset images show the representative of the signals from TOM20 and Rab5 under control (left panel) and H 2 O 2 -treated condition (right panel). Scale bars, 10 μm. ( C ) Subcellular fractionation of cytosolic (Cyto) and mitochondrial (Mito) fractions from WT and Alsin -/- iPSC-sMN treated with either PBS (control) or 200 μM H 2 O 2 for 1 hours at 37°C. Protein samples were loaded onto SDS-PAGE and imunoblotted with Rab5, tubulin (cytoplasmic loading control), and TOM20 antibodies by Western blot. ( D ) Cytosolic fractions were prepared as in from WT and Alsin -/- iPSC-sMN challenged with 200 μM H 2 O 2 for 1 hours at 37μC. Densitometric quantification of cytosolic cytochrome c from Western blot probed cytochrome c antibody. Data were collected from three independent experiments. Y-axis corresponds to normalized ratio intensity of experimental to loading control (* p <0.05; ** p <0.005).

Article Snippet: Cells were immunostained with corresponding primary: anti-rabbit Rabenosyn-5/ZFYVE20 (Sigma Aldrich: HPA044878), anti-mouse EEA1 (BD Biosciences: 610457), anti-rabbit TOM20 (Santa Cruz Biotechnology: sc-11415), anti-rabbit APPL1 (Abcam: ab59592), anti-mouse Rab5 (BD Biosciences: 610724), anti-mouse cytochrome c (Abcam: ab6311), and anti-rabbit Alsin (Novus Biological: NBP2-14284) antibodies.

Techniques: Control, Fractionation, SDS Page, Western Blot

In normal condition, mitochondria (Mito, red) are elongated and tubular (top left). Rab5 (green) are localized on early endosomes (EE) to assemble Rab5 machinery for endosomal maturation and membrane trafficking. At steady state, we observed some EE transiently making contacts with mitochondria. During oxidative stress (eg. laser- or chemically-induced) that leads to MOMP, mitochondria undergo a dramatic morphological transformation into rounded and swollen structures (top right). This is accompanied by an increase in Rab5-positive endosomes forming MCS with mitochondria of less than <5 nm. Release of apoptotic signal such as cytochrome c from mitochondria into the cytosol is associated with Rab5 translocation to the OMM, which is involved in blocking cytochrome c release and caspase activation. The recruitment and activation of Rab5 on mitochondria depend on the Rab5 GEF Alsin (blue), resulting in a selective recruitment of Rabenosyn-5 (light green), in order to regulate apoptosis and confer cytoprotection.

Journal: bioRxiv

Article Title: Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria

doi: 10.1101/200428

Figure Lengend Snippet: In normal condition, mitochondria (Mito, red) are elongated and tubular (top left). Rab5 (green) are localized on early endosomes (EE) to assemble Rab5 machinery for endosomal maturation and membrane trafficking. At steady state, we observed some EE transiently making contacts with mitochondria. During oxidative stress (eg. laser- or chemically-induced) that leads to MOMP, mitochondria undergo a dramatic morphological transformation into rounded and swollen structures (top right). This is accompanied by an increase in Rab5-positive endosomes forming MCS with mitochondria of less than <5 nm. Release of apoptotic signal such as cytochrome c from mitochondria into the cytosol is associated with Rab5 translocation to the OMM, which is involved in blocking cytochrome c release and caspase activation. The recruitment and activation of Rab5 on mitochondria depend on the Rab5 GEF Alsin (blue), resulting in a selective recruitment of Rabenosyn-5 (light green), in order to regulate apoptosis and confer cytoprotection.

Article Snippet: Cells were immunostained with corresponding primary: anti-rabbit Rabenosyn-5/ZFYVE20 (Sigma Aldrich: HPA044878), anti-mouse EEA1 (BD Biosciences: 610457), anti-rabbit TOM20 (Santa Cruz Biotechnology: sc-11415), anti-rabbit APPL1 (Abcam: ab59592), anti-mouse Rab5 (BD Biosciences: 610724), anti-mouse cytochrome c (Abcam: ab6311), and anti-rabbit Alsin (Novus Biological: NBP2-14284) antibodies.

Techniques: Membrane, Transformation Assay, Translocation Assay, Blocking Assay, Activation Assay